By watching this presentation, you will learn key strategies for:
- PFAS analysis with targeted and non-targeted LC/MS/MS approaches
- Workflow development on wastewater with EPA draft method
- A comprehensive workflow development for PFAS analysis
Event Overview
Per- and polyfluoroalkyl substances (PFAS) pose an increasing threat to the environment, animals, and human beings due to extreme chemical stability and bioaccumulation potential. PFAS analysis is a complex problem that is best addressed with complementary targeted and non-targeted approaches. A comprehensive LC/MS/MS workflow was developed for the PFAS analysis in wastewater based on the existing EPA draft Method 1633 with additional PFAS of varying size and functional groups. Strategies for sensitivity will also be discussed.
However, targeted assays only cover a small fraction of PFAS. Therefore, non-targeted methods and software are very important to understand the health risks of PFAS. Comprehensive software, FluoroMatch suite, covers peak picking, annotation, homologous series detection, assigning confidence, and PFAS data visualization. Workflows taking advantage of the Agilent iterative exclusion approach are shown, as well as data-independent acquisition (DIA) workflows.
These presentations were recorded during ASMS 2023 so if you weren’t able to attend in person here's your chance to watch them on-demand. The presentations are followed by a Q&A.
What you need to know:
Format: On-demand
Duration: Approximately 50 minutes
Presenters:
Emily Parry
LC/MS Application Scientist, Agilent
Emily Parry has focused her career on developing methods of measurement for environmental contaminants in both human and environmental matrices. She brings ten years of laboratory experience using both targeted and untargeted techniques to her role as LCMS Application Scientist at Agilent Technologies. Prior to joining Agilent, she worked as a researcher at the Environmental Protection Agency (EPA) and the Public Health Institute. She received her Ph.D. degree in Environmental Chemistry from the University of California, Davis, where she was the recipient of the National Science Foundation Fellowship.
Jeremy Koelmel
Researcher at Krystal Pollitt Laboratory, Yale University, and CEO of Innovative Omics
Dr. Jeremy Koelmel leads a team of software developers as CEO of Innovative Omics and as a researcher at Yale University. He has led the development of over ten software and written 60 publications in the last six years covering intelligent data acquisition, lipidomics, PFAS analysis, polymers, and mass spectral visualizations. He has worked closely with Agilent Technologies, including helping to develop Lipid Annotator and Agilent's lipidomics solution, and on the open-source FluoroMatch software, which is explained in the presentation.
Tarun Anumol
Environmental Segment Manager, Agilent
Tarun Anumol is the Director for the Global Environment Market for Agilent Technologies. Previously, he was an LC-MS applications scientist at Agilent, focused on developing analytical methods for trace contaminants on LC-MS/MS and LC-Q/TOF in environmental and food matrices. Tarun has a strong history of working in the environmental testing industry, with a background in technical and applied science and experience in water treatment, environmental monitoring, and advanced analytical chemistry.
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