Agilent Technologies has produced an application note proving equivalency of the Agilent 1200 Series Binary LC and the Agilent 1260 Infinity II LC for the analysis of local anesthetics.
Introduction
Instrument-to-instrument method transfer is an important topic for all laboratories, and is especially important in the pharmaceutical industry. This Application Note shows the seamless transfer of a conventional LC method for the analysis of local anesthetics from an Agilent 1200 Series Binary LC to an Agilent 1260 Infinity II LC, and demonstrates that equivalent results in terms of retention time and resolution are obtained. In addition, the conventional LC method is transferred to UHPLC conditions on the 1260 Infinity II LC, which offers the possibility of increasing peak resolution, while enabling time and solvent savings.
Results and Discussion
The analysis of the local anesthetics benzocaine, lidocaine, prilocaine, and tetracaine using a conventional LC method on a 1200 Series Binary LC is performed. The method is transferred to a 1260 Infinity II LC including a 1260 Infinity II Binary Pump for proof of equivalency. In addition, the conventional LC method is transferred to UHPLC conditions using the 1260 Infinity II LC. Excellent retention time and area precision were achieved.
Conclusions
The transfer of the conventional LC method for the analysis of the local anesthetics benzocaine, lidocaine, prilocaine, and tetracaine from an Agilent 1200 Series Binary LC to an Agilent 1260 Infinity II LC showed a maximum retention time deviation of 0.3 %, while comparable peak resolution was obtained. This proves the equivalency of the 1260 Infinity II LC compared to the 1200 Series Binary LC
for the conventional LC analysis of local anesthetics. With its pressure range of up to 600 bar, the 1260 Infinity II LC enables UHPLC analyses using Agilent InfinityLab Poroshell columns. By transfer of the
conventional LC method for the analysis of local anesthetics to UHPLC conditions, the analysis time and solvent use were greatly reduced.
