Shimadzu has produced an application note describing a single LC-MS/MS method for the simultaneous determination of 18 mycotoxins in food safety.
Introduction
LC-MS/MS is the technique most commonly employed for mycotoxin quantitation in order to achieve the necessary low reporting limits in complex food and feed matrices. Due to the wide range of physical and chemical properties of mycotoxins, different LC-MS/MS methods are typically developed for small groups of compounds with similar properties to ensure that sufficient chromatographic separation is achieved for differential quantitation of isomeric species, which in turn compromise the total throughput of screening analysis.
Method
This article describes a single LC-MS/MS method that has been developed for the simultaneous determination of 18 mycotoxins in food safety, including: Aflatoxins (B1, B2, G1, G2), Fumonisins (B1, B2, B3), Ochratoxin A (OTA), Trichothecenes (3-acetyldeoxynivalenol (3-AcDON), 15-acetyldeoxynivalenol (15-AcDON), Deoxynivalenol (DON), Diaceteoxyscripanol (DAS), Fusarenon-X (FUS X), HT-2, Neosolaninol (NEO), Nivalenol (NIV), T2, Zearalenone (ZON). The analysis was short, provided chromatographic resolution of isomeric species and fulfilled the reporting limits dictated by Regulation (EC) No. 1886/2006 (amended by (EC) No. 1126/2007).
By downloading this application note you will:
- learn that such large panel of mycotoxin analysis is actually possible using the LCMS-8060 system.
