Restek has produced an application note demonstrating how the Raptor FluoroPhenyl column is a good choice for vitamin D analysis as opposed to C18 columns.
Introduction
Vitamin D analysis has increased dramatically in clinical practice due to its association with multiple human diseases including cancer, rheumatoid arthritis, and diabetes. It also has an important role in calcium and phosphorous absorption in relation to bone strength, so there is growing concern about the prevalence of vitamin D deficiency worldwide. Accurate LC analysis of vitamin D epimers is critical for quantitative determination of the bioactive forms of vitamin D.
Experimental
LC-MS/MS is a common technique for vitamin D analysis; however, because the biomarkers and their epimers are isobaric, chromatographic separation is essential for accurate quantitation. Many published methods employ a C18 column, but its utility is limited by incomplete resolution of the biomarkers and their epimers as well as by analysis times of 10 minutes or more. The Raptor FluoroPhenyl column is a better choice as it provides alternate selectivity to a C18 column and completely separates the isobaric epimers and biomarkers in just 5 minutes.
Conclusion
Raptor FluoroPhenyl columns improve LC analysis of vitamin D epimers, reliably providing accurate measurement of vitamin D content for disease and deficiency investigation and monitoring.
By using the Raptor FluroPhenyl column you will achieve:
• 5-minute analysis time allowing more samples per day.
• Complete separation of vitamin D epimers ensuring accurate results.
• A reduction in cost per sample due to the long-lasting, rugged nature of the LC columns
