Biotage has produced an application note describing the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns.
Introduction
ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.
This application note describes an effective and efficient ISOLUTE SLE+ protocol optimized for the 1 mL capacity column format. The simple sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQ of 50 ppb, suitable for screening applications.
Method
The analytes under investigation in this application were Amphetamine, Bendiocarb, Methamphetamine, Propanil, MDMA, Chlorothalonil, Atrazine, utabarbital, Secobarbital, Ketamine, Malathion, Phenobarbital, Cocaine, Methadone, THC, Bifenthrin, Diazepam, Nitrazepam, Midazolam, Clonazepam, Estazolam, Alprazolam and Triazolam. A protocol was developed that allowed the simultaneous extraction of these various drugs of abuse. In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.
Results
Blank liver supernatant was spiked at 1000 ppb for recovery testing. The protocol described offered reproducible recovery with most RSD values <10%.
