Fundamentals of HPLC

by | Mar 27, 2023

Learn about the basics and fundamentals of HPLC including instrumentation, measurements and parameters, chemistry, and quantitation.

FHPLCAre you new to HPLC, or do you supervise new users of HPLC? 'Fundamentals of HPLC' is taught by Tom Jupille, a world-class expert in HPLC, and represents an opportunity to give new users a firm grounding in the fundamentals of HPLC economically and with minimum disruption to work schedules. “Fundamentals of HPLC” is intended for beginner chromatographers. No hands-on LC experience is required, but you should have a solid grasp of basic chemistry.

‘Fundamentals of HPLC’ covers the essentials that every scientist needs in order to make effective use of liquid chromatography instrumentation, including:

  • The basics of the chromatographic process
  • Terms and definitions of chromatography
  • How HPLC systems work
  • Practical tips on preventive maintenance
  • Principles of separation chemistry
  • Why “reversed phase” is the standard separation technique
  • The meaning of “gradients” and why they are used
  • How to get the best quantitation
  • and much more

The course is broken down into 5 main sections covering:

  • General overview
  • Intrumentation
  • Measurements and parameters
  • Chemistry
  • Quantitation

In total across these five sections there are 18 modules structured in a logical manner to aid the learning experience, and each module has a complementary quiz. By correctly answering all of the quiz questions for all of the modules you will be able to download your certificate of completion.

By taking this online course you will acquire a good understanding of the fundamentals of HPLC and get a better knowledge of the key concepts involved. The course is aimed at new users, as well as managers, supervisors, auditors, reviewers, etc. who use information generated by HPLC but do not necessarily do chromatography themselves.

The course price is US$ 189 for one user for 12 months of access. Multi-user pricing is also available, as are site licences for larger teams with multiple course requirements.

Analytical Training Solutions, brought to you by Separation Science, is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique education resource for analytical chemists. Currently we offer HPLC training, LC-MS training, GC training and GC-MS training.

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