Routine analysis of therapeutic protein N-glycosylation has become important in biopharma discovery, development and QC labs. This presentation will briefly review recent advances in automated sample preparation and ultra-high performance HILIC separations of fluorescent labeled released N-glycans. These developments have greatly improved the speed and ease of analysis. Some analysts now find that the greatest remaining challenge is N-glycan peak assignment, particularly when dealing with new sample types or unexpected glycan peaks.
Fortunately, the Agilent LC/MSD XT, based on single quadrupole technology, is available to provide a new level of confidence for routine glycan analysis. This highly sensitive, stackable mass selective detector can be hyphenated downstream of a fluorescence detector in order to greatly facilitate peak assignment and even allow relative quantitation of coeluting structures. Furthermore, glycans can be fragmented in this instrument to distinguish between structural isomers. This presentation will demonstrate results from the analysis of a range of monoclonal antibodies and related products.
Separation Science, in collaboration with Agilent Technologies, has developed an eSeminar covering the analysis of biotherapeutics and biosimilars.
This presentation is given by Oscar Potter (R&D Scientist, Agilent Technologies).
By attending this presentation you will learn about...
- Recap on automated sample preparation and HILIC separation of released, labeled N-glycan analysis
- Learn how a mass selective detector can enhance routine glycan analysis by confirming peak identities and quantifying coeluting structures
