Thermo Fisher Scientific has produced an application note to demonstrate the robustness of the Thermo Scientific™ TSQ Quantis™ triple quadrupole mass spectrometer by analysis of immunosuppressant drugs in whole blood for clinical research.
Introduction
Mass spectrometry is a popular choice of technology in clinical research for the quantitation of immunosuppressant drugs in whole blood. A sensitive and robust instrument is required for reliable quantitation workflows. In this study, we demonstrate the TSQ Quantis triple quadrupole mass spectrometer for this application.
Test of Robustness
The daily sequence of samples consisted of an initial set of eight calibrators. This was followed by repeated sets of five controls and 20 blank blood samples. A set of calibrators was inserted approximately halfway through the total sequence and again at the end. The total number of samples per sequence was approximately 300. The sequence was repeated for five consecutive days. Peak areas of the internal standards and calculated concentration of the mid-level control were monitored for stability.
Conclusion
- The TSQ Quantis triple quadrupole MS demonstrated robust, reliable and consistent performance for more than 1500 samples over five days with no need for maintenance.
- Calibrators and controls maintain precision and accuracy demonstrating reliability.
- The quantitative workflow exhibited reproducible results on a sensitive and robust platform suitable for analysis of immunosuppressant drugs in blood for clinical research.
