In the pursuit of accurate and sensitive LC-MS/MS analysis, particularly at low analyte concentrations, sample preparation is a crucial step that can make or break the success of an assay. Separation Science recently engaged in an insightful discussion with Dr. Stephanie Marin, the Global Clinical Market Development Manager at Phenomenex, to explore the intricacies of sample preparation workflows for low-level LC-MS/MS analysis.
With extensive experience in developing and validating clinical assays, Dr. Marin brings a wealth of knowledge in liquid chromatography and mass spectrometry to the conversation. In this interview, she provides an in-depth comparison of various extraction techniques, shedding light on their suitability in the context of endocrine testing.
In this interview, Dr. Marin provides insights in response to the following questions:
- Could you provide a brief overview of liquid-liquid extraction (LLE), solid-liquid extraction (SLE), and solid-phase extraction (SPE)?
- How do LLE, SLE, and SPE compare in terms of efficiency, recovery, and reproducibility for endocrine testing?
- How do you see the future of sample prep evolving, particularly for low-level endocrine testing?
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